Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATF2 antibody
See all ATF2 primary antibodies
DescriptionRabbit polyclonal to ATF2
SpecificityThis antibody detects endogenous levels of ATF2 protein around Threonine 73 or 55.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide around the phosphorylation site of Threonine 73 or 55 (Human)
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
Ab31483, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma tissue byImmunohistochemistry.
Left image: Using ab31483.
Right image: The same antibody preincubated with synthesized peptide.
All lanes : Anti-ATF2 antibody (ab31483) at 1/500 dilution
Lane 1 : Extracts from LOVO cells (5-30ug) treated with ab31483.
Lane 2 : Extracts from LOVO cells (5-30ug) treated using the same antibody preincubated with
All lanes : goat-anti-rabbit IgG-AP-conjugate.
Predicted band size: 54 kDa
ab31483 has not yet been referenced specifically in any publications.