Anti-ATF2 antibody (ab31483)
Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATF2 antibody
See all ATF2 primary antibodies -
Description
Rabbit polyclonal to ATF2 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of ATF2 protein around Threonine 73 or 55. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide around the phosphorylation site of Threonine 73 or 55 (Human)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
Tissue specificity
Abundant expression seen in the brain. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
Post-translational
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
see all -
Alternative names
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody (ab31483)Ab31483, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma tissue byImmunohistochemistry.
Left image: Using ab31483.
Right image: The same antibody preincubated with synthesized peptide. -
Western blot - Anti-ATF2 antibody (ab31483)All lanes : Anti-ATF2 antibody (ab31483) at 1/500 dilution
Lane 1 : Extracts from LOVO cells (5-30ug) treated with ab31483.
Lane 2 : Extracts from LOVO cells (5-30ug) treated using the same antibody preincubated with
synthesized peptide.
Secondary
All lanes : goat-anti-rabbit IgG-AP-conjugate.
Predicted band size: 54 kDa
Protocols
Datasheets and documents
References (0)
ab31483 has not yet been referenced specifically in any publications.
