Product nameAnti-ATF2 antibody
See all ATF2 primary antibodies
DescriptionRabbit polyclonal to ATF2
SpecificityATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53.
Tested applicationsSuitable for: WB, IHC-P, ELISA, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
The antiserum was produced against synthesized non-phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
Corresponding phospho antibody
Our Abpromise guarantee covers the use of ab47476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 52 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
All lanes : Anti-ATF2 antibody (ab47476) at 1/500 dilution
Lane 1 : extracts from HeLa cells.
Lane 2 : extracts from HeLa cells preincubated with immunizing peptide
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.
This product has been referenced in:
- Zhou Y et al. Ligustilide attenuates nitric oxide-induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways. J Cell Mol Med 23:3357-3368 (2019). Read more (PubMed: 30770640) »
- Liu F et al. The role of NF-?B-mediated JNK pathway in cognitive impairment in a rat model of sleep apnea. J Thorac Dis 10:6921-6931 (2018). Read more (PubMed: 30746238) »