Key features and details
- Goat polyclonal to ATF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATF2 antibody
See all ATF2 primary antibodies
DescriptionGoat polyclonal to ATF2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Dog
- WB: HepG2, K562 and HeLa nuclear lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
Primary incubation 1 hour at room temperature.
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
ab809 has been referenced in 1 publication.
- Karim AS et al. Nox2 is a mediator of ischemia reperfusion injury. Am J Transplant 15:2888-99 (2015). PubMed: 26104383