Product nameAnti-ATF2 antibody [E242]
See all ATF2 primary antibodies
DescriptionRabbit monoclonal [E242] to ATF2
Specificityab32061 recognises ATF-2 (activating transcription factor-2, CRE-BP1). It does not cross-react with other ATF family members
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human ATF2 aa 50-150 (N terminal). The exact sequence is proprietary.
- HeLa whole cell lysate (ab150035) and human carcinoma
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).|
|ICC||1/250 - 1/500.|
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
ab32061 (purified) at 1/500 dilution immunoprecipitating ATF2 in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): HeLa whole cell lysate 10µg
Lane 2 (+): ab32061 + HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-ATF2 antibody [E242] (ab32061) at 1/10000 dilution + HeLa cell lysate
Predicted band size: 54 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human carcinoma using ab32061 at a dilution of 1/50