• Product name
    Anti-ATF2 antibody [E242]
    See all ATF2 primary antibodies
  • Description
    Rabbit monoclonal [E242] to ATF2
  • Host species
  • Specificity
    ab32061 recognises ATF-2 (activating transcription factor-2, CRE-BP1). It does not cross-react with other ATF family members
  • Tested applications
    Suitable for: WB, ICC, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATF2 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa whole cell lysate (ab150035) and human carcinoma
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab32061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
ICC 1/250 - 1/500.
IHC-P 1/100.
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
    • Tissue specificity
      Abundant expression seen in the brain.
    • Sequence similarities
      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • Post-translational
      Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating transcription factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      see all


    • ab32061 (purified) at 1/500 dilution immunoprecipitating ATF2 in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
      Lane 1 (input): HeLa whole cell lysate 10µg
      Lane 2 (+): ab32061 + HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution. 

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-ATF2 antibody [E242] (ab32061) at 1/10000 dilution + HeLa cell lysate

      Predicted band size: 54 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded human carcinoma using ab32061 at a dilution of 1/50


    This product has been referenced in:
    • Li Q  et al. ATF2 translation is induced under chemotherapeutic drug-mediated cellular stress via an IRES-dependent mechanism in human hepatic cancer Bel7402 cells. Oncol Lett 12:4795-4802 (2016). Read more (PubMed: 28105187) »
    See 1 Publication for this product

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