• Product name

    Anti-ATF2 antibody [E243]
    See all ATF2 primary antibodies
  • Description

    Rabbit monoclonal [E243] to ATF2
  • Host species

  • Specificity

    This antibody recognises ATF2, but does not cross react with other ATF family members.
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATF2 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Breast carcinoma tissue. Flow Cyt: HeLa cells. IP: HeLa cell lysate ChIP: Jurkat cells
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
IP 1/60.
Flow Cyt 1/2000.


  • Function

    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • Tissue specificity

    Abundant expression seen in the brain.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • Post-translational

    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all


  • Anti-ATF2 antibody [E243] (ab32160) at 1/10000 dilution + HeLa cell lysate

    Predicted band size: 54 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Chromatin was prepared from Jurkat (TPA and Ionomycin treated or not) cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab32160 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • ab32160 (purified) at 1/1000 dilution (2.284 µg/ml) immunoprecipitating ATF2 in HeLa whole cell lysate.
    Lane 3 (-): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µgab32160 & HeLa whole cell lysateRabbit monoclonal IgG (ab172730) instead of ab32160 in HeLa whole cell lysate
    For western blotting, Rabbit TureBlot: Anti-Rabbit IgG HRP was used as the secondary antibody at 1/1500 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATF2 (red) with ab32160 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.


This product has been referenced in:

  • Ma J  et al. SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression. J Exp Clin Cancer Res 37:145 (2018). Read more (PubMed: 29996942) »
  • Wu X  et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun 7:13498 (2016). WB ; Human . Read more (PubMed: 27853137) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Dog Cell lysate - whole cell (canine dermal fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
canine dermal fibroblasts
Blocking step
(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. 令 中野

Verified customer

Submitted Jun 17 2019

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