Anti-ATF2 (phospho T71 + T53) antibody (ab28812)
Key features and details
- Rabbit polyclonal to ATF2 (phospho T71 + T53)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF2 (phospho T71 + T53) antibody
See all ATF2 primary antibodies -
Description
Rabbit polyclonal to ATF2 (phospho T71 + T53) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATF2 (phospho T53 + T71).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Corresponding non-phospho antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
Tissue specificity
Abundant expression seen in the brain. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
Post-translational
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
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Alternative names
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
Images
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Ab28812, at a dilution of 1/50, staining ATF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using the ATF2 antibody
Right image: using the same antibody preincubated with synthesized phospho peptide. -
All lanes : Anti-ATF2 (phospho T71 + T53) antibody (ab28812) at 1/1000 dilution
Lane 1 : untreated HeLa cells (5-30ug lysate).
Lane 2 : HeLa cells treated with UV light (5-30ug lysate).
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab28812 has been referenced in 1 publication.
- White SJ et al. Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress. J Cell Physiol 226:2841-8 (2011). WB . PubMed: 21302282