Key features and details
- Mouse monoclonal [5A9B9] to ATF3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ATF3 antibody [5A9B9]
See all ATF3 primary antibodies
DescriptionMouse monoclonal [5A9B9] to ATF3
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant full length protein corresponding to Human ATF3 aa 1-181. (Expressed in E.coli).
MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQ NKHLCHRMSSALESVTVSDRPLGVSITKAEVAPEEDERKKRRRERNKIAA AKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQHLIYMLNLHR PTCIVRAQNGRTPEDERNLFIQQIKEGTLQS
Database link: P18847
- WB: Recombinant human ATF3 protein; ATF3 (aa: 1-181)-hIgGFc transfected HEK-293 cell lysate. Flow: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500.|
|Flow Cyt||1/200 - 1/400.|
FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
All lanes : Anti-ATF3 antibody [5A9B9] (ab233797) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ATF3 (AA: 1-181)-hIgGFc transfected HEK-293 cell lysate
Anti-ATF3 antibody [5A9B9] (ab233797) at 1/500 dilution + Recombinant human ATF3 protein
Expected MW is 46.5 kDa
Flow cytometric analysis of A549 (human lung carcinoma cell line) cells labeling ATF3 using ab233797 at 1/200 dilution (green) compared with a negative control (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233797 has not yet been referenced specifically in any publications.