Product nameAnti-ATF3 antibody
See all ATF3 primary antibodies
DescriptionRabbit polyclonal to ATF3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human
- Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869) can be used as a positive control in WB. Mouse heart cell lysate; Mouse skeletal muscle cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/200.|
FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
All lanes : Anti-ATF3 antibody (ab180842) at 1/1000 dilution
Lane 1 : mouse heart tissue lysate
Lane 2 : mouse skeletal muscle tissue lysate
All lanes : Goat Anti-Rabbit IgG (H+L) HRP conjugate
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling ATF3 with ab180842 at 1/100 dilution. A Goat Anti-Rabbit IgG (H+L) FITC conjugate secondary antibody has been used. DAPI has been used for nuclear staining.