Anti-ATF3 antibody (ab216569)
Key features and details
- Rabbit polyclonal to ATF3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATF3 antibody
See all ATF3 primary antibodies -
Description
Rabbit polyclonal to ATF3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ATF3 aa 80-130 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:EVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELK A
Database link: P18847 -
Positive control
- Human colon carcinoma and colon cancer tissues; Rat brain tissue; Mouse lung and brain lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab216569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. (or 1/50 – 1/200 if using a fluorescent secondary antibody). |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 21 kDa. |
Target
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Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 467 Human
- Entrez Gene: 11910 Mouse
- Entrez Gene: 25389 Rat
- Omim: 603148 Human
- SwissProt: P18847 Human
- SwissProt: Q60765 Mouse
- SwissProt: P29596 Rat
- Unigene: 460 Human
see all -
Alternative names
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon cancer tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution.
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Western blot - Anti-ATF3 antibody (ab216569)All lanes : Anti-ATF3 antibody (ab216569) at 1/300 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 21 kDa10% gel
Protocols
Datasheets and documents
References (2)
ab216569 has been referenced in 2 publications.
- Grigore F et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
- Yao J et al. Single-cell transcriptomic analysis in a mouse model deciphers cell transition states in the multistep development of esophageal cancer. Nat Commun 11:3715 (2020). PubMed: 32709844
