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    atf3-antibody-ab216569.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-ATF3 antibody (ab216569)

  • Datasheet
Reviews (3) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
  • Western blot - Anti-ATF3 antibody (ab216569)

Key features and details

  • Rabbit polyclonal to ATF3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ATF3 antibody
    See all ATF3 primary antibodies
  • Description

    Rabbit polyclonal to ATF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATF3 aa 80-130 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    EVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELK A


    Database link: P18847
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon carcinoma and colon cancer tissues; Rat brain tissue; Mouse lung and brain lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse lung normal tissue lysate - total protein (ab29297)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)

Applications

Our Abpromise guarantee covers the use of ab216569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

(or 1/50 – 1/200 if using a fluorescent secondary antibody).

WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • Function

    This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P18847 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 467 Human
    • Entrez Gene: 11910 Mouse
    • Entrez Gene: 25389 Rat
    • Omim: 603148 Human
    • SwissProt: P18847 Human
    • SwissProt: Q60765 Mouse
    • SwissProt: P29596 Rat
    • Unigene: 460 Human
    • Unigene: 2706 Mouse
    • Unigene: 9664 Rat
    see all
  • Alternative names

    • Activating transcription factor 3 antibody
    • ATF3 antibody
    • ATF3_HUMAN antibody
    • ATF3deltaZip2 antibody
    • ATF3deltaZip2c antibody
    • ATF3deltaZip3 antibody
    • cAMP dependent transcription factor ATF3 antibody
    • cAMP-dependent transcription factor ATF-3 antibody
    • Cyclic AMP dependent transcription factor ATF3 antibody
    • Cyclic AMP-dependent transcription factor ATF-3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon cancer tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution.

  • Western blot - Anti-ATF3 antibody (ab216569)
    Western blot - Anti-ATF3 antibody (ab216569)
    All lanes : Anti-ATF3 antibody (ab216569) at 1/300 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 21 kDa



    10% gel

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab216569? Please let us know so that we can cite the reference in this datasheet.

    ab216569 has been referenced in 2 publications.

    • Grigore F  et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
    • Yao J  et al. Single-cell transcriptomic analysis in a mouse model deciphers cell transition states in the multistep development of esophageal cancer. Nat Commun 11:3715 (2020). PubMed: 32709844

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-ATF3 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monodelphis domestica Cell lysate - whole cell (Cortex)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    Cortex
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Jelena Ban

    Verified customer

    Submitted May 01 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATF3 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver (MCD diet NASH model))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - 3% H2O2
    Specification
    Liver (MCD diet NASH model)
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ATF3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Permeabilization
    Yes - 0.5 % Triton
    Specification
    A549
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2018

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