Anti-ATF3 antibody (ab216569)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ATF3 antibody
See all ATF3 primary antibodies -
Description
Rabbit polyclonal to ATF3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ATF3 aa 80-130 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:EVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELK A
Database link: P18847 -
Positive control
- Human colon carcinoma and colon cancer tissues; Rat brain tissue; Mouse lung and brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab216569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. (or 1/50 – 1/200 if using a fluorescent secondary antibody). |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 21 kDa. |
Target
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Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 467 Human
- Entrez Gene: 11910 Mouse
- Entrez Gene: 25389 Rat
- Omim: 603148 Human
- SwissProt: P18847 Human
- SwissProt: Q60765 Mouse
- SwissProt: P29596 Rat
- Unigene: 460 Human
see all -
Alternative names
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon cancer tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody (ab216569)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ATF3 with ab216569 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution.
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Western blot - Anti-ATF3 antibody (ab216569)All lanes : Anti-ATF3 antibody (ab216569) at 1/300 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 21 kDa10% gel
Datasheets and documents
References
ab216569 has not yet been referenced specifically in any publications.
