Anti-ATF3 [CL1685] antibody (ab191513)
Key features and details
- Mouse monoclonal [CL1685] to ATF3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ATF3 [CL1685] antibody
See all ATF3 primary antibodies -
Description
Mouse monoclonal [CL1685] to ATF3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human ATF3 aa 1-150.
Database link: P18847-1 -
Epitope
ab191513 recognizes an epitope corresponding to amino acids 16-30 (ASAIVPCLSPPGSLV) of Human ATF3 as determined by overlapping synthetic peptides. -
Positive control
- ICC/IF: RH-30 cells. IHC-P: Human placenta, fallopian tube, duodenum and stomach cancer tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
CL1685 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation: PFA/Triton X-100 |
Notes |
---|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation: PFA/Triton X-100 |
Target
-
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 515266 Cow
- Entrez Gene: 467 Human
- Entrez Gene: 11910 Mouse
- Entrez Gene: 25389 Rat
- Omim: 603148 Human
- SwissProt: Q2KII1 Cow
- SwissProt: P18847 Human
- SwissProt: Q60765 Mouse
see all -
Alternative names
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human placenta (Left) and tonsil tissues (Right) labeling ATF3 with ab191513. Antigen retrieval using HIER pH6. The data is produced following IHC standard protocol. Positive staining was observed on Human placenta.
-
PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for ATF3 (Green) using ab191513 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
-
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
-
Immunohistochemical analysis of paraffin-embedded Human fallopain tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
-
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
-
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab191513 has been referenced in 4 publications.
- Tang X et al. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest 127:4314-4325 (2017). PubMed: 29083321
- Li J et al. ATF3 suppresses ESCC via downregulation of ID1. Oncol Lett 12:1642-1648 (2016). IHC . PubMed: 27602100
- Keilhoff G et al. Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med 11:1685-1699 (2016). IHC-FoFr ; Rat . PubMed: 27168790
- Taniue K et al. ASBEL-TCF3 complex is required for the tumorigenicity of colorectal cancer cells. Proc Natl Acad Sci U S A N/A:N/A (2016). PubMed: 27791078