Key features and details
- Mouse monoclonal [CL1685] to ATF3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ATF3 [CL1685] antibody
See all ATF3 primary antibodies
DescriptionMouse monoclonal [CL1685] to ATF3
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Epitopeab191513 recognizes an epitope corresponding to amino acids 16-30 (ASAIVPCLSPPGSLV) of Human ATF3 as determined by overlapping synthetic peptides.
- ICC/IF: RH-30 cells. IHC-P: Human placenta, fallopian tube, duodenum and stomach cancer tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation: PFA/Triton X-100
FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for ATF3 (Green) using ab191513 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
Immunohistochemical analysis of paraffin-embedded Human fallopain tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling AFT3 with ab191513 antibody at a dilution of 1/200.
ab191513 has been referenced in 4 publications.
- Tang X et al. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest 127:4314-4325 (2017). PubMed: 29083321
- Li J et al. ATF3 suppresses ESCC via downregulation of ID1. Oncol Lett 12:1642-1648 (2016). IHC . PubMed: 27602100
- Keilhoff G et al. Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Exp Ther Med 11:1685-1699 (2016). IHC-FoFr ; Rat . PubMed: 27168790
- Taniue K et al. ASBEL-TCF3 complex is required for the tumorigenicity of colorectal cancer cells. Proc Natl Acad Sci U S A N/A:N/A (2016). PubMed: 27791078