Overview

  • Product name

    Anti-ATF5 antibody [EPR18286] (Alexa Fluor® 647)
    See all ATF5 primary antibodies
  • Description

    Rabbit monoclonal [EPR18286] to ATF5 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human ATF5 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y2D1

  • Positive control

    • ICC/IF: NIH3T3 cells. Flow Cyt: NIH3T3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.
ICC/IF 1/1000.

This product gave a positive signal in NIH3T3 cells fixed with 100% methanol (5 min)

Target

  • Relevance

    ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • Activating transcription factor 5 alpha/beta antibody
    • Activating transcription factor 5 antibody
    • Activating transcription factor X antibody
    • AFTA antibody
    • ATF 5 antibody
    • ATF 7 antibody
    • ATF7 antibody
    • ATFX antibody
    • BZIP protein ATF7 antibody
    • cAMP dependent transcription factor ATF 5 antibody
    • cAMP dependent transcription factor ATF5 antibody
    • Cyclic AMP dependent transcription factor ATF 5 antibody
    • Cyclic AMP dependent transcription factor ATF5 antibody
    • FLJ34666 antibody
    • HMFN0395 antibody
    • MGC102397 antibody
    • NAP1 antibody
    • NRIF3 associated protein antibody
    • ODA 10 antibody
    • Transcription factor ATFx antibody
    • Transcription factor like protein ODA 10 antibody
    • Transcription factor like protein ODA10 antibody
    see all

Images

  • Overlay histogram showing NIH3T3 cells stained with ab225336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225336, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • ab225336 staining ATF5 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225336 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225336 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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