Overview

  • Product name

    Anti-ATF5 antibody [EPR18286] (Phycoerythrin)
    See all ATF5 primary antibodies
  • Description

    Rabbit monoclonal [EPR18286] to ATF5 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human ATF5 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y2D1

  • Positive control

    • Flow Cyt: NIH3T3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Relevance

    ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • Activating transcription factor 5 alpha/beta antibody
    • Activating transcription factor 5 antibody
    • Activating transcription factor X antibody
    • AFTA antibody
    • ATF 5 antibody
    • ATF 7 antibody
    • ATF7 antibody
    • ATFX antibody
    • BZIP protein ATF7 antibody
    • cAMP dependent transcription factor ATF 5 antibody
    • cAMP dependent transcription factor ATF5 antibody
    • Cyclic AMP dependent transcription factor ATF 5 antibody
    • Cyclic AMP dependent transcription factor ATF5 antibody
    • FLJ34666 antibody
    • HMFN0395 antibody
    • MGC102397 antibody
    • NAP1 antibody
    • NRIF3 associated protein antibody
    • ODA 10 antibody
    • Transcription factor ATFx antibody
    • Transcription factor like protein ODA 10 antibody
    • Transcription factor like protein ODA10 antibody
    see all

Images

  • Overlay histogram showing NIH3T3 cells stained with ab225335 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225335, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

References

ab225335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225335.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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