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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-ATF6 antibody (ab134561)

  • Datasheet
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Western blot - Anti-ATF6 antibody (ab134561)
  • Immunoprecipitation - Anti-ATF6 antibody (ab134561)

Key features and details

  • Rabbit polyclonal to ATF6
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human ATF6 protein (ab131846)
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Human ATF6 knockout HeLa cell line (ab261800)

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Overview

  • Product name

    Anti-ATF6 antibody
    See all ATF6 primary antibodies
  • Description

    Rabbit polyclonal to ATF6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 620-670 of Human ATF6 (NP_031374.2).

  • Positive control

    • Whole cell lysate from HeLa, 293T and Jurkat cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa membrane extract lysate (ab29547)
    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant Human ATF6 protein (ab131846)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rabbit
Chimpanzee
Gorilla
Elephant
Application Abreviews Notes
WB (1)
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Domain

    The basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • Post-translational
    modifications

    During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
    N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
    Phosphorylated in vitro by MAPK14/P38MAPK.
  • Cellular localization

    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Target information above from: UniProt accession P18850 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22926 Human
    • Omim: 605537 Human
    • SwissProt: P18850 Human
    • Unigene: 492740 Human
    • Alternative names

      • Activating transcription factor 6 alpha antibody
      • Activating transcription factor 6 antibody
      • ATF 6 antibody
      • ATF6 alpha antibody
      • ATF6 antibody
      • ATF6-alpha antibody
      • ATF6A antibody
      • ATF6A_HUMAN antibody
      • cAMP dependent transcription factor ATF 6 alpha antibody
      • cAMP-dependent transcription factor ATF-6 alpha antibody
      • Cyclic AMP dependent transcription factor ATF 6 alpha antibody
      • DKFZp686P2194 antibody
      • ESTM49 antibody
      • FLJ21663 antibody
      • Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
      • Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
      see all

    Images

    • Western blot - Anti-ATF6 antibody (ab134561)
      Western blot - Anti-ATF6 antibody (ab134561)
      All lanes : Anti-ATF6 antibody (ab134561) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 74 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-ATF6 antibody (ab134561)
      Immunoprecipitation - Anti-ATF6 antibody (ab134561)
      Detection of ATF6 by Western Blot of Immunprecipitate.
      Immunoprecipitation of ATF6 in HeLa whole cell lysate, using ab134561 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      For the subsequent Western blot, ab134561 was used at 1 µg/ml.
      The lysate in lane 2 is precipitated with an IgG control antibody.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab134561? Please let us know so that we can cite the reference in this datasheet.

    ab134561 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-ATF6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prosate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Prosate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2017

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