Anti-ATF6 antibody (ab134561)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ATF6 antibody
See all ATF6 primary antibodies -
Description
Rabbit polyclonal to ATF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Gorilla, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 620-670 of Human ATF6 (NP_031374.2).
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Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 74 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Domain
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. -
Post-translational
modificationsDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK. -
Cellular localization
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22926 Human
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 492740 Human
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Alternative names
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
Images
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Western blot - Anti-ATF6 antibody (ab134561)All lanes : Anti-ATF6 antibody (ab134561) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ATF6 antibody (ab134561)Detection of ATF6 by Western Blot of Immunprecipitate.
Immunoprecipitation of ATF6 in HeLa whole cell lysate, using ab134561 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
For the subsequent Western blot, ab134561 was used at 1 µg/ml.
The lysate in lane 2 is precipitated with an IgG control antibody.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
ab134561 has not yet been referenced specifically in any publications.
