Anti-ATF6 antibody (ab134561)
Key features and details
- Rabbit polyclonal to ATF6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF6 antibody
See all ATF6 primary antibodies -
Description
Rabbit polyclonal to ATF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 620-670 of Human ATF6 (NP_031374.2).
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Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rabbit
Chimpanzee
Gorilla
Elephant
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 74 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Domain
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. -
Post-translational
modificationsDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK. -
Cellular localization
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22926 Human
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 492740 Human
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Alternative names
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
Images
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Western blot - Anti-ATF6 antibody (ab134561)All lanes : Anti-ATF6 antibody (ab134561) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ATF6 antibody (ab134561)Detection of ATF6 by Western Blot of Immunprecipitate.
Immunoprecipitation of ATF6 in HeLa whole cell lysate, using ab134561 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
For the subsequent Western blot, ab134561 was used at 1 µg/ml.
The lysate in lane 2 is precipitated with an IgG control antibody.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
References (0)
ab134561 has not yet been referenced specifically in any publications.
