Overview

  • Product name

  • Description

    Rabbit polyclonal to ATF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATF6 aa 300-350 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    VGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLK K


    Database link: P18850

  • Positive control

    • WB: Mouse pancreas lysates IHC :Rat brain tissue. ICC/IF: U-251 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab203119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 75 kDa.
ICC/IF 1/200.

Target

  • Function

    Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Domain

    The basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • Post-translational
    modifications

    During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
    N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
    Phosphorylated in vitro by MAPK14/P38MAPK.
  • Cellular localization

    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor 6 alpha antibody
    • Activating transcription factor 6 antibody
    • ATF 6 antibody
    • ATF6 alpha antibody
    • ATF6 antibody
    • ATF6-alpha antibody
    • ATF6A antibody
    • ATF6A_HUMAN antibody
    • cAMP dependent transcription factor ATF 6 alpha antibody
    • cAMP-dependent transcription factor ATF-6 alpha antibody
    • Cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • DKFZp686P2194 antibody
    • ESTM49 antibody
    • FLJ21663 antibody
    • Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
    see all

Images

  • Anti-ATF6 antibody (ab203119) at 1/300 dilution + mouse pancreas lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution

    Predicted band size: 75 kDa

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue sections labeling ATF6 with ab203119 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunocytochemistry/ Immunofluorescence analysis of U-251 cells labeling ATF6 with ab203119 at 1/200 (diluted in PBS and incubated for 2 hours at 37°C). Goat Anti-Rabbit IgG (H+L) FITC conjugated antibody was used as the secondary antibody. Nuclei were stained with DAPI (blue).

References

This product has been referenced in:

  • Pandey VK  et al. Activation of PERK-eIF2a-ATF4 pathway contributes to diabetic hepatotoxicity: Attenuation of ER stress by Morin. Cell Signal 59:41-52 (2019). Read more (PubMed: 30877037) »
  • Wei J  et al. Homer1a Attenuates Endoplasmic Reticulum Stress-Induced Mitochondrial Stress After Ischemic Reperfusion Injury by Inhibiting the PERK Pathway. Front Cell Neurosci 13:101 (2019). Read more (PubMed: 30930751) »
See all 17 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 28 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 28 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 03 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 06 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP - prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
30 µg
Specification
LNCaP - prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 21 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 21 2017

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