Anti-ATF6 antibody (ab203119)
Key features and details
- Rabbit polyclonal to ATF6
- Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ATF6 antibody
See all ATF6 primary antibodies -
Description
Rabbit polyclonal to ATF6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ATF6 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P18850 -
Positive control
- WB: huvec, raji, jurkat, a549, thp-1, 293t and du145 cells ICC/IF: U-251 cells IHC: HeLa cells Flow-Cyt: Jurkat cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
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WB | (1) |
1/100 - 1/1000. Predicted molecular weight: 75 kDa.
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Flow Cyt (Intra) |
1/100.
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ICC/IF | (3) |
1/200.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
WB
1/100 - 1/1000. Predicted molecular weight: 75 kDa. |
Flow Cyt (Intra)
1/100. |
ICC/IF
1/200. |
Target
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Function
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Domain
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. -
Post-translational
modificationsDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK. -
Cellular localization
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22926 Human
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 492740 Human
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Alternative names
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of U-251 cells labeling ATF6 with ab203119 at 1/200 (diluted in PBS and incubated for 2 hours at 37°C). Goat Anti-Rabbit IgG (H+L) FITC conjugated antibody was used as the secondary antibody. Nuclei were stained with DAPI (blue).
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Immunohistochemical analysis of HeLa cells stained for ATF6 using ab203119 at a dilution of 1:100.
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All lanes : Anti-ATF6 antibody (ab203119) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lane 5 : thp-1 cell lysate
Lane 6 : 293T cell lysate
Lane 7 : du145 cell lysate
Secondary
All lanes : Conjugated secondary at 1/2000 dilution
Predicted band size: 75 kDa -
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ATF6 Antibody (ab203119) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab203119 has been referenced in 56 publications.
- Yu R et al. ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice. Andrologia 54:e14350 (2022). PubMed: 34904262
- Thongnak L et al. The combination of dapagliflozin and statins ameliorates renal injury through attenuating the activation of inflammasome-mediated autophagy in insulin-resistant rats. J Biochem Mol Toxicol 36:e22978 (2022). PubMed: 34939712
- Yuan Y et al. Endoplasmic reticulum stress promotes the release of exosomal PD-L1 from head and neck cancer cells and facilitates M2 macrophage polarization. Cell Commun Signal 20:12 (2022). PubMed: 35090495
- Wang Z et al. The Endoplasmic Reticulum-Stressed Head and Neck Squamous Cell Carcinoma Cells Induced Exosomal miR-424-5p Inhibits Angiogenesis and Migration of Humanumbilical Vein Endothelial Cells Through LAMC1-Mediated Wnt/β-Catenin Signaling Pathway. Cell Transplant 31:9636897221083549 (2022). PubMed: 35315295
- Shen J et al. Homocysteine facilitates endoplasmic reticulum stress and apoptosis of hepatocytes by suppressing ERO1α expression via cooperation between DNMT1 and G9a. Cell Biol Int 46:1236-1248 (2022). PubMed: 35347798