Product nameAnti-ATF6 antibody
See all ATF6 primary antibodies
DescriptionRabbit polyclonal to ATF6
SpecificityBased on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as the it recognizes a peptide sequence from near the amino terminus. We have not tested stressed cells to see if the 50kDa cleavage product can be detected.
Tested applicationsSuitable for: ICC, ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ATF6 (N terminal). 17 a.a. immunogen somewhere between a.a. 25-75
- MDA MB 361 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity purified
Our Abpromise guarantee covers the use of ab37149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Only Peptide ELISA has been tested.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).|
FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
DomainThe basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
modificationsDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK.
Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
- Information by UniProt
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
All lanes : Anti-ATF6 antibody (ab37149) at 0.5 µg
Lane 1 : Untreated HeLa Cell Lysate
Lane 2 : Tunicamycin-treated-2mg HeLa Cell Lysate
Lane 3 : Tunicamycin-treated-20mg HeLa Cell Lysate
Predicted band size: 75 kDa
ab37149 staining human Hella cells by ICC/IF. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit (ab175471) diluted at 1/1000.
A20 (mouse reticulum sarcoma cell line) cells stained for ATF6 using ab37149 at 10 µg/ml in ICC.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ATF6 (green) using ab37149 at 1/100 dilution in ICC/IF, followed by Goat anti-rabbit Alexa Fluor® 488.
This product has been referenced in:
- Liang H et al. Hypoxia induces miR-153 through the IRE1a-XBP1 pathway to fine tune the HIF1a/VEGFA axis in breast cancer angiogenesis. Oncogene N/A:N/A (2018). WB . Read more (PubMed: 29367761) »
- Li PC et al. Seawater inhalation induces acute lung injury via ROS generation and the endoplasmic reticulum stress pathway. Int J Mol Med 41:2505-2516 (2018). Read more (PubMed: 29436612) »