Key features and details
- Rabbit polyclonal to ATF6 - Carboxyterminal end
- Suitable for: ICC/IF, ICC, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATF6 antibody - Carboxyterminal end
See all ATF6 primary antibodies
DescriptionRabbit polyclonal to ATF6 - Carboxyterminal end
Specificityab62576 only reacts with only ATF6 alpha.
Tested applicationsSuitable for: ICC/IF, ICC, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
A 16 amino acid peptide from near the carboxy terminus of human ATF6
- ICC/IF: MCF7 cells. WB: EL4 cell lysate. IHC-P: Human breast tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|ICC||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
DomainThe basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
modificationsDuring unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK.
Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
- Information by UniProt
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.
Paraffin-embedded human breast tissue stained for ATF6 using ab62576 at 5 µg/ml in immunohistochemical analysis.
ab62576 at 10µg/ml staining AFT6 in MCF7 cells by Immunocytochemistry.
Lane 1 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 0.5 µg/ml
Lane 2 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 1 µg/ml
All lanes : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence of ATF6 in MCF7 cells using ab62576 at 10 ug/ml.
ab62576 has been referenced in 2 publications.
- Ma AG et al. PSMD4 regulates the malignancy of esophageal cancer cells by suppressing endoplasmic reticulum stress. Kaohsiung J Med Sci 35:591-597 (2019). PubMed: 31162820
- Liu J et al. High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A. Biomed Res Int 2016:4130834 (2016). WB ; Mouse . PubMed: 27579314