Key features and details
- Rabbit polyclonal to ATF7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATF7 antibody
See all ATF7 primary antibodies
DescriptionRabbit polyclonal to ATF7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
Recombinant fragment (His-T7-tag) corresponding to Mouse ATF7 aa 50-270. N-terminal tags. Expressed in E. coli.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-QTPTPTRFLKNCE EVGLFNELASSFEHEFKKASDDDEKKGAAGPLDMSLPSTPDIKIKEEEPV EVDSSPPDSPASSPCSPPLKEKEVTTKPVVISTPTPTIVRPGSLPLHLGY DPLHPTLPSPTSVITQAPPSNRQIGSPTGSLPLVMHLANGQTMPMLPGPP VQMPSVISLARPVSMVPNIPGIPGPPVNNSGSISPSGHPMPSEAKMRLKA TLTHQVSS
Database link: Q8R0S1
- IHC-P: Mouse spleen, liver, pancreas, intestine, ovary and kidney tissue. WB: Recombinant mouse ATF7 protein. Rat stomach tissue lysate. SGC7901 and HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionPlays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
Tissue specificityExpressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsOn EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
Cellular localizationNucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
- Information by UniProt
- 1110012F10Rik antibody
- 9430065F09Rik antibody
- Activating transcription factor 7 antibody
All lanes : Anti-ATF7 antibody (ab231786) at 2 µg/ml
Lane 1 : SGC7901 cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Rat stomach tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded mouse intestine tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse ovary tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse spleen tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for ATF7 with ab231786 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-ATF7 antibody (ab231786) at 2 µg/ml + Recombinant mouse ATF7 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231786 has not yet been referenced specifically in any publications.