• Product name

  • Description

    Rabbit polyclonal to ATF7
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 444-494 (ATAPSNGLSV RSAAEAVATS VLTQMASQRT ELSMPIQSHV IMTPQSQSAG R) of human ATF7 (CAA40483.1).

  • Positive control

    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab87844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
      Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
    • Tissue specificity

      Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
    • Sequence similarities

      Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • Post-translational

      On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
      Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
    • Cellular localization

      Nucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1110012F10Rik antibody
      • 9430065F09Rik antibody
      • Activating transcription factor 7 antibody
      • AI549878 antibody
      • ATF7 antibody
      • Atf7 protein antibody
      • ATF7_HUMAN antibody
      • ATFA antibody
      • C130020M04Rik antibody
      • cAMP-dependent transcription factor ATF-7 antibody
      • Cyclic AMP-dependent transcription factor ATF 7 antibody
      • Cyclic AMP-dependent transcription factor ATF-7 antibody
      • MGC31554 antibody
      • MGC57182 antibody
      • Transcription factor ATF A antibody
      • Transcription factor ATF-A antibody
      see all


    • All lanes : Anti-ATF7 antibody (ab87844) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 53 kDa
      Observed band size: 63 kDa
      why is the actual band size different from the predicted?

      Exposure time: 30 seconds
    • Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
      Developed using the ECL technique, with an exposure time of 10 seconds.


    ab87844 has not yet been referenced specifically in any publications.

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