Key features and details
- Rabbit polyclonal to ATF7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATF7 antibody
See all ATF7 primary antibodies
DescriptionRabbit polyclonal to ATF7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to ATF7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab87844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
Tissue specificityExpressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsOn EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
Cellular localizationNucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
- Information by UniProt
- 1110012F10Rik antibody
- 9430065F09Rik antibody
- Activating transcription factor 7 antibody
All lanes : Anti-ATF7 antibody (ab87844) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
Developed using the ECL technique, with an exposure time of 10 seconds.
ab87844 has not yet been referenced specifically in any publications.