Key features and details
- Rabbit polyclonal to ATG10
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG10 antibody
See all ATG10 primary antibodies
DescriptionRabbit polyclonal to ATG10
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human ATG10. The exact sequence is proprietary.
Database link: Q9H0Y0
- WB: HEK-293T whole cell lysate. IP: SK-N-SH whole cell extract.
Previously labelled as Apg10.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IP||1/100 - 1/500.|
FunctionE2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Sequence similaritiesBelongs to the ATG10 family.
- Information by UniProt
- Apg 10 antibody
- APG 10L antibody
- APG10 antibody
ATG10 was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell lysate with 5 μg of ab229461. Western blot was performed from the immunoprecipitate using ab229461.
Lane 1: Control IgG in SK-N-SH whole cell lysate.
Lane 2: ab229461 IP in SK-N-SH whole cell lysate.
All lanes : Anti-ATG10 antibody (ab229461) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ATG10-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229461 has not yet been referenced specifically in any publications.