Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human ATG10. The exact sequence is proprietary.
    Database link: Q9H0Y0

  • Positive control

    • WB: HEK-293T whole cell lysate. IP: SK-N-SH whole cell extract.
  • General notes

    Previously labelled as Apg10. 

Properties

Applications

Our Abpromise guarantee covers the use of ab229461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP 1/100 - 1/500.

Target

  • Function

    E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
  • Sequence similarities

    Belongs to the ATG10 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apg 10 antibody
    • APG 10L antibody
    • APG10 antibody
    • APG10 autophagy 10 like antibody
    • APG10 like antibody
    • APG10, S. cerevisiae, homolog of antibody
    • APG10-like antibody
    • APG10L antibody
    • ATG 10 antibody
    • ATG10 antibody
    • ATG10 autophagy related 10 homolog (S. cerevisiae) antibody
    • ATG10 autophagy related 10 homolog antibody
    • ATG10_HUMAN antibody
    • autophagy 10, S. cerevisiae, homolog of antibody
    • Autophagy related protein 10 antibody
    • Autophagy-related protein 10 antibody
    • DKFZP586I0418 antibody
    • FLJ13954 antibody
    • pp12616 antibody
    • Ubiquitin-like-conjugating enzyme ATG10 antibody
    see all

Images

  • ATG10 was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell lysate with 5 μg of ab229461. Western blot was performed from the immunoprecipitate using ab229461.

    Lane 1: Control IgG in SK-N-SH whole cell lysate.
    Lane 2: ab229461 IP in SK-N-SH whole cell lysate.

  • All lanes : Anti-ATG10 antibody (ab229461) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : ATG10-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 25 kDa



    12% SDS-PAGE gel.

References

ab229461 has not yet been referenced specifically in any publications.

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