Key features and details
- Rabbit polyclonal to ATG10 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG10 antibody - C-terminal
See all ATG10 primary antibodies
DescriptionRabbit polyclonal to ATG10 - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ATG10 (C terminal). The exact sequence is proprietary.
Database link: Q9H0Y0
- WB: SK-N-SH and LoVo whole cell extracts. IHC-P: Human ovarian cancer tissue.
Previously labelled as Apg10.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
FunctionE2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Sequence similaritiesBelongs to the ATG10 family.
- Information by UniProt
- Apg 10 antibody
- APG 10L antibody
- APG10 antibody
All lanes : Anti-ATG10 antibody - C-terminal (ab229728) at 1/500 dilution
Lane 1 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 2 : LoVo (human colorectal adenocarcinoma cell line) whole cell extract
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
12% SDS-PAGE gel.
Paraffin-embedded human ovarian cancer tissue stained for ATG10 with ab229728 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229728 has not yet been referenced specifically in any publications.