Recombinant Anti-ATG10 antibody [EPR4804] (ab124711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4804] to ATG10
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATG10 antibody [EPR4804]
See all ATG10 primary antibodies -
Description
Rabbit monoclonal [EPR4804] to ATG10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATG10 aa 200-300 (C terminal).
Database link: Q9H0Y0 -
Positive control
- WB: HeLa, K562, and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4804 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
Target
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Function
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. -
Sequence similarities
Belongs to the ATG10 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 83734 Human
- Omim: 610800 Human
- SwissProt: Q9H0Y0 Human
- Unigene: 713698 Human
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Alternative names
- Apg 10 antibody
- APG 10L antibody
- APG10 antibody
see all
Images
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All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ATG10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab124711 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124711 Anti-ATG10 antibody [EPR4804] was shown to specifically react with ATG10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265682 (knockout cell lysate ab258786) was used. Wild-type and ATG10 knockout samples were subjected to SDS-PAGE. ab124711 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab124711 has been referenced in 14 publications.
- Peng J et al. The m6A methyltransferase METTL3 affects autophagy and progression of nasopharyngeal carcinoma by regulating the stability of lncRNA ZFAS1. Infect Agent Cancer 17:1 (2022). PubMed: 34980191
- Xu X et al. A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer. Aging (Albany NY) 14:4858-4873 (2022). PubMed: 35680565
- Polo P et al. Robustness of the Autophagy Pathway to Somatic Copy Number Losses. Cells 11:N/A (2022). PubMed: 35681458
- Pavel M et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
- Zhu Y et al. SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by ß-catenin and Beclin1/autophagy signaling in colorectal cancer. Cell Death Dis 12:449 (2021). PubMed: 33953166