Product nameAnti-ATG10 antibody [EPR4804]
See all ATG10 primary antibodies
DescriptionRabbit monoclonal [EPR4804] to ATG10
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ATG10 aa 200-300 (C terminal).
Database link: Q9H0Y0
- HeLa, K562, and 293T cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Apg10.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IP||1/10 - 1/100.|
FunctionE2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Sequence similaritiesBelongs to the ATG10 family.
- Information by UniProt
- Apg 10 antibody
- APG 10L antibody
- APG10 antibody
All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Runwal G et al. LC3-positive structures are prominent in autophagy-deficient cells. Sci Rep 9:10147 (2019). Read more (PubMed: 31300716) »
- Puri C et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB . Read more (PubMed: 29634932) »