Overview

  • Product name

    Anti-ATG10 antibody [EPR4804]
    See all ATG10 primary antibodies
  • Description

    Rabbit monoclonal [EPR4804] to ATG10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG10 aa 200-300 (C terminal).
    Database link: Q9H0Y0

  • Positive control

    • HeLa, K562, and 293T cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Apg10. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
    • Sequence similarities

      Belongs to the ATG10 family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apg 10 antibody
      • APG 10L antibody
      • APG10 antibody
      • APG10 autophagy 10 like antibody
      • APG10 like antibody
      • APG10, S. cerevisiae, homolog of antibody
      • APG10-like antibody
      • APG10L antibody
      • ATG 10 antibody
      • ATG10 antibody
      • ATG10 autophagy related 10 homolog (S. cerevisiae) antibody
      • ATG10 autophagy related 10 homolog antibody
      • ATG10_HUMAN antibody
      • autophagy 10, S. cerevisiae, homolog of antibody
      • Autophagy related protein 10 antibody
      • Autophagy-related protein 10 antibody
      • DKFZP586I0418 antibody
      • FLJ13954 antibody
      • pp12616 antibody
      • Ubiquitin-like-conjugating enzyme ATG10 antibody
      see all

    Images

    • All lanes : Anti-ATG10 antibody [EPR4804] (ab124711) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Runwal G  et al. LC3-positive structures are prominent in autophagy-deficient cells. Sci Rep 9:10147 (2019). Read more (PubMed: 31300716) »
    • Puri C  et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB . Read more (PubMed: 29634932) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124711.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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