Key features and details
- Rabbit polyclonal to ATG101
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATG101 antibody
See all ATG101 primary antibodies
DescriptionRabbit polyclonal to ATG101
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
16 amino acid peptide near the center of human ATG101 (GenBank accession no. NP_001092143).
- Human liver tissue lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionAutophagy factor required for autophagosome formation.
Sequence similaritiesBelongs to the ATG101 family.
Cellular localizationCytoplasm. Preautophagosomal structure. Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
- Information by UniProt
- ATG101 antibody
- Atg13-interacting protein antibody
- ATGA1_HUMAN antibody
Lane 1 : Anti-ATG101 antibody (ab105387) at 1 µg/ml
Lane 2 : Anti-ATG101 antibody (ab105387) at 2 µg/ml
All lanes : human liver tissue lysate
Lysates/proteins at 15 µg/ml per lane.
Predicted band size: 25 kDa
Immunofluorescence of ATG101 in mouse liver tissue with ab105387 at 20 ?g/mL.
Immunohistochemistry of ATG101 in mouse liver with ab105387 antibody at 5 µg/ml
ab105387 has been referenced in 4 publications.
- You M et al. 10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells. Int Immunopharmacol 84:106501 (2020). PubMed: 32311670
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Ho WY et al. The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy. Autophagy 15:827-842 (2019). PubMed: 30669939
- Pyo KE et al. ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation. Cell Rep 25:2878-2890.e4 (2018). PubMed: 30517873