Anti-ATG101 antibody (ab229235)
Key features and details
- Rabbit polyclonal to ATG101
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG101 antibody
See all ATG101 primary antibodies -
Description
Rabbit polyclonal to ATG101 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Rhesus monkey -
Immunogen
Synthetic peptide within Human ATG101 (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9BSB4 -
Positive control
- WB: NCI-H1299 whole cell extract. IHC-P: A549 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
Target
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Function
Autophagy factor required for autophagosome formation. -
Sequence similarities
Belongs to the ATG101 family. -
Cellular localization
Cytoplasm. Preautophagosomal structure. Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. - Information by UniProt
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Database links
- Entrez Gene: 513049 Cow
- Entrez Gene: 60673 Human
- Entrez Gene: 68118 Mouse
- Entrez Gene: 300240 Rat
- Entrez Gene: 696954 Rhesus monkey
- Entrez Gene: 446504 Xenopus laevis
- Entrez Gene: 567680 Zebrafish
- SwissProt: Q9BSB4 Human
see all -
Alternative names
- ATG101 antibody
- Atg13-interacting protein antibody
- ATGA1_HUMAN antibody
see all
Images
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Anti-ATG101 antibody (ab229235) at 1/1000 dilution + NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Paraffin-embedded A549 (human lung carcinoma cell line) xenograft tissue stained for ATG101 using ab229235 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229235 has been referenced in 1 publication.
- Zhang ZJ et al. Comprehensive Analysis of the Potential Immune-Related Biomarker ATG101 that Regulates Apoptosis of Cholangiocarcinoma Cells After Photodynamic Therapy. Front Pharmacol 13:857774 (2022). PubMed: 35592424