Key features and details
- Rabbit polyclonal to ATG101
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG101 antibody
See all ATG101 primary antibodies
DescriptionRabbit polyclonal to ATG101
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
Synthetic peptide within Human ATG101 (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9BSB4
- WB: NCI-H1299 whole cell extract. IHC-P: A549 xenograft tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
FunctionAutophagy factor required for autophagosome formation.
Sequence similaritiesBelongs to the ATG101 family.
Cellular localizationCytoplasm. Preautophagosomal structure. Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
- Information by UniProt
- ATG101 antibody
- Atg13-interacting protein antibody
- ATGA1_HUMAN antibody
Anti-ATG101 antibody (ab229235) at 1/1000 dilution + NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 25 kDa
12% SDS-PAGE gel.
Paraffin-embedded A549 (human lung carcinoma cell line) xenograft tissue stained for ATG101 using ab229235 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229235 has not yet been referenced specifically in any publications.