Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG12
  • Host species

    Rabbit
  • Specificity

    ab155589 detects free ATG12 and ATG12 conjugated to Atg5.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ATG12 aa 1-187. The exact sequence is proprietary.

  • Positive control

    • 293T, A431, NIH3T3 and Rat brain whole cell lysates; Human U87 xenograft tissue; HeLa cells untreated or treated with Tunicamycin.

Properties

Applications

Our Abpromise guarantee covers the use of ab155589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the ATG12 family.
  • Domain

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apg12 antibody
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Images

  • Anti-ATG12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Anti-ATG12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • All lanes : Anti-ATG12 antibody (ab155589) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling ATG12 with ab155589 at 1/500 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ATG12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
    Cells were fixed in 4% paraformaldehyde for 15 minutes.

References

This product has been referenced in:

  • Ouchida AT  et al. SET protein accumulation prevents cell death in head and neck squamous cell carcinoma through regulation of redox state and autophagy. Biochim Biophys Acta Mol Cell Res 1866:623-637 (2019). Read more (PubMed: 30658075) »
  • Li X  et al. Luteolin suppresses lipopolysaccharide-induced cardiomyocyte hypertrophy and autophagy in vitro. Mol Med Rep 19:1551-1560 (2019). Read more (PubMed: 30628693) »
See all 11 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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