Overview

  • Product name
  • Description
    Rabbit polyclonal to ATG12
  • Host species
    Rabbit
  • Specificity
    ab155589 detects free ATG12 and ATG12 conjugated to Atg5.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ATG12 aa 1-187. The exact sequence is proprietary.

  • Positive control
    • 293T, A431, NIH3T3 and Rat brain whole cell lysates; Human U87 xenograft tissue; HeLa cells untreated or treated with Tunicamycin.

Properties

Applications

Our Abpromise guarantee covers the use of ab155589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 15 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the ATG12 family.
  • Domain
    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apg12 antibody
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Images

  • Anti-ATG12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Anti-ATG12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • All lanes : Anti-ATG12 antibody (ab155589) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling ATG12 with ab155589 at 1/500 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ATG12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
    Cells were fixed in 4% paraformaldehyde for 15 minutes.

References

This product has been referenced in:
  • Bao X  et al. Inhibition of LCMR1 and ATG12 by demethylation-activated miR-570-3p is involved in the anti-metastasis effects of metformin on human osteosarcoma. Cell Death Dis 9:611 (2018). Read more (PubMed: 29795113) »
  • Maueröder C  et al. Capability of Neutrophils to Form NETs Is Not Directly Influenced by a CMA-Targeting Peptide. Front Immunol 8:16 (2017). Read more (PubMed: 28191006) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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