Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG12 (N terminal). (NP_001264712.1).
    Database link: O94817

  • Positive control

    • WB: HeLa cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab240900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF 1/100.

Target

  • Function

    Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the ATG12 family.
  • Domain

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apg12 antibody
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATG12 with ab240900 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Rabbit ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue), used at a 1/5000 dilution for 5 minutes at RT.

    A: DAPI

    B: ATG12

    C: Composite.

  • Anti-ATG12 antibody (ab240900) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Exposure time: 12 minutes


    Primary incubation for 1 hour at room temperature.

    Blocking buffer: 5% milk + TBST.

References

ab240900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240900.
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