Key features and details
- Rabbit polyclonal to ATG12
- Suitable for: WB, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-ATG12 antibody
See all ATG12 primary antibodies
DescriptionRabbit polyclonal to ATG12
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant full length protein corresponding to Saccharomyces cerevisiae ATG12.
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesPurified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Our Abpromise guarantee covers the use of ab4752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 22.1 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
FunctionUbiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
Sequence similaritiesBelongs to the ATG12 family.
DomainShares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
- Information by UniProt
- Apg12 antibody
- APG12-like antibody
- APG12L antibody
ab4752 generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125). Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes
ab4752 has been referenced in 1 publication.
- Crews L et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . PubMed: 20174468