Overview

  • Product name

    Anti-ATG12 antibody [EPR4800] - BSA and Azide free
    See all ATG12 primary antibodies
  • Description

    Rabbit monoclonal [EPR4800] to ATG12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ATG12 aa 100-200 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab247876 is the carrier-free version of ab109491. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247876 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the ATG12 family.
    • Domain

      Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apg12 antibody
      • APG12-like antibody
      • APG12L antibody
      • ATG12 antibody
      • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
      • ATG12 autophagy related 12 homolog antibody
      • ATG12_HUMAN antibody
      • Autophagy 12 antibody
      • Autophagy-related protein 12 antibody
      • FBR93 antibody
      • HAPG12 antibody
      • Ubiquitin-like protein ATG12 antibody
      see all

    References

    ab247876 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab247876.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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