Overview

  • Product name

    Anti-ATG12 antibody - N-terminal
    See all ATG12 primary antibodies
  • Description

    Rabbit polyclonal to ATG12 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG12 (N terminal). An adjuvant has been used to orchestrate/boost the immune response.
    Database link: O94817

  • Positive control

    • Flow Cyt: SH-SY5Y cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

Target

  • Function

    Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the ATG12 family.
  • Domain

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apg12 antibody
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Images

  • Flow cytometric analysis of methanol-fixed, 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling ATG12 with ab228494 at 2 µg per 106 cells (black) compared with a Rabbit IgG Control (white). Donkey anti-Rabbit PE at 1/100 dilution was used as the secondary antibody.

References

ab228494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228494.
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