Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG14L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the center of Human ATG14 (NP_055739).

  • Positive control

    • Human small intestine tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab139727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
  • Sequence similarities

    Belongs to the Barkor family.
  • Domain

    The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4832427M01 antibody
    • ATG14 antibody
    • Atg14L antibody
    • Autophagy-related protein 14-like protein antibody
    • BAKOR_HUMAN antibody
    • Barkor antibody
    • Beclin 1-associated autophagy-related key regulator antibody
    • D14Ertd114e antibody
    • D14Ertd436e antibody
    • KIAA0831 antibody
    • mCG_6911 antibody
    see all

Images

  • Immunofluorescence of ATG14L in Human small intestine tissue with ab139727 at 20 ug/mL.
  • Lane 1 : Anti-ATG14L antibody (ab139727) at 1 µg/ml
    Lane 2 : Anti-ATG14L antibody (ab139727) at 2 µg/ml

    All lanes : Human small intestine tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 55 kDa

References

This product has been referenced in:

  • Qiu S  et al. The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells. Biomed Pharmacother 109:1841-1850 (2019). Read more (PubMed: 30551439) »
  • Losier TT  et al. AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles. Cell Rep 26:2150-2165.e5 (2019). Read more (PubMed: 30784596) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Mouse Cell lysate - other (Mouse embryonic Fibroblast)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
Mouse embryonic Fibroblast

Abcam user community

Verified customer

Submitted Jul 20 2018

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