Key features and details
- Rabbit polyclonal to ATG14L
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG14L antibody
See all ATG14L primary antibodies
DescriptionRabbit polyclonal to ATG14L
SpecificityAt least three isoforms of ATG14 are known to exist; ab189949 will detect all three isoforms.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ATG14L (internal sequence). The peptide corresponds to 18 amino acids.
Database link: Q6ZNE5
- Human skin tissue; Human small intestine tissue; Human small intestine cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionRequired for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
Sequence similaritiesBelongs to the Barkor family.
DomainThe coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
Cellular localizationCytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
- Information by UniProt
- 4832427M01 antibody
- ATG14 antibody
- Atg14L antibody
Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human skin tissue labeling ATG14L with ab189949 at 5µg/ml.
Lane 1 : Anti-ATG14L antibody (ab189949) at 1 µg/ml
Lane 2 : Anti-ATG14L antibody (ab189949) at 2 µg/ml
All lanes : Human small intestine cell lysate
Predicted band size: 55 kDa
ab189949 has not yet been referenced specifically in any publications.