Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG14L
  • Host species

    Rabbit
  • Specificity

    At least three isoforms of ATG14 are known to exist; ab189949 will detect all three isoforms.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG14L (internal sequence). The peptide corresponds to 18 amino acids.
    Database link: Q6ZNE5

  • Positive control

    • Human skin tissue; Human small intestine tissue; Human small intestine cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
  • Sequence similarities

    Belongs to the Barkor family.
  • Domain

    The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4832427M01 antibody
    • ATG14 antibody
    • Atg14L antibody
    • Autophagy-related protein 14-like protein antibody
    • BAKOR_HUMAN antibody
    • Barkor antibody
    • Beclin 1-associated autophagy-related key regulator antibody
    • D14Ertd114e antibody
    • D14Ertd436e antibody
    • KIAA0831 antibody
    • mCG_6911 antibody
    see all

Images

  • Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human skin tissue labeling ATG14L with ab189949 at 5µg/ml.

  • Lane 1 : Anti-ATG14L antibody (ab189949) at 1 µg/ml
    Lane 2 : Anti-ATG14L antibody (ab189949) at 2 µg/ml

    All lanes : Human small intestine cell lysate

    Predicted band size: 55 kDa

References

ab189949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189949.
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