Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG14L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human ATG14L (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q6ZNE5

  • Positive control

    • IHC-P: HeLa xenograft tissue. WB: HeLa, HepG2, HEK-293T and RD whole cell extracts. Mouse and rat brain extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
  • Sequence similarities

    Belongs to the Barkor family.
  • Domain

    The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4832427M01 antibody
    • ATG14 antibody
    • Atg14L antibody
    • Autophagy-related protein 14-like protein antibody
    • BAKOR_HUMAN antibody
    • Barkor antibody
    • Beclin 1-associated autophagy-related key regulator antibody
    • D14Ertd114e antibody
    • D14Ertd436e antibody
    • KIAA0831 antibody
    • mCG_6911 antibody
    see all

Images

  • All lanes : Anti-ATG14L antibody (ab227849) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : RD whole cell extract
    Lane 3 : Mouse brain extract
    Lane 4 : Rat brain extract

    Lysates/proteins at 60 µg per lane.

    Predicted band size: 55 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded HeLa xenograft tissue stained for ATG14L using ab227849 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-ATG14L antibody (ab227849) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

References

ab227849 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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