Anti-ATG14L antibody - N-terminal (ab173943)

Overview

  • Product name
    Anti-ATG14L antibody - N-terminal
    See all ATG14L primary antibodies
  • Description
    Rabbit polyclonal to ATG14L - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ATG14L aa 62-92 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ZNE5

  • Positive control
    • Mouse heart tissue lysate; Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab173943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 55 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
  • Sequence similarities
    Belongs to the Barkor family.
  • Domain
    The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4832427M01 antibody
    • ATG14 antibody
    • Atg14L antibody
    • Autophagy-related protein 14-like protein antibody
    • BAKOR_HUMAN antibody
    • Barkor antibody
    • Beclin 1-associated autophagy-related key regulator antibody
    • D14Ertd114e antibody
    • D14Ertd436e antibody
    • KIAA0831 antibody
    • mCG_6911 antibody
    see all

Images

  • Anti-ATG14L antibody - N-terminal (ab173943) at 1/50 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size: 55 kDa

  • Flow cytometric analysis of Jurkat cells (bottom histogram) labeling ATG14L with ab173943 at 1/10 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab173943 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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Submitted Jun 14 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted May 24 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

Submitted May 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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