Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG16L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG16L1 (internal sequence). 18 amino acids.
    Database link: Q676U5

  • Positive control

    • Human placenta and uterus tissue. HeLa cells. HeLa whole cell lysate (ab150035).

Properties

Applications

Our Abpromise guarantee covers the use of ab188642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease

    Inflammatory bowel disease 10
  • Sequence similarities

    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization

    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Form

    There are 4 isoforms produced by alternative splicing.
  • Alternative names

    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • Lane 1 : Anti-ATG16L1 antibody (ab188642) at 0.25 µg/ml
    Lane 2 : Anti-ATG16L1 antibody (ab188642) at 0.5 µg/ml

    All lanes : HeLa cell lysate

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.

  • Immunocytochemical/ Immunofluorescence analysis of HeLa cells labeling ATG16L1 using ab188642 at 4 µg/ml.

  • Immunocytochemical analysis of HeLa cells labeling ATG16L1 using ab188642 at 2 µg/ml.

References

This product has been referenced in:

  • Zhang K  et al. PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell Death Dis 9:312 (2018). Read more (PubMed: 29472524) »
  • Liu H  et al. Oxidative Stress Mediates Microcystin-LR-Induced Endoplasmic Reticulum Stress and Autophagy in KK-1 Cells and C57BL/6 Mice Ovaries. Front Physiol 9:1058 (2018). Read more (PubMed: 30131715) »
See all 2 Publications for this product

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