Key features and details
- Rabbit polyclonal to ATG16L1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATG16L1 antibody
See all ATG16L1 primary antibodies
DescriptionRabbit polyclonal to ATG16L1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human ATG16L1 (internal sequence). 18 amino acids.
Database link: Q676U5
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 4 µg/ml.|
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
Involvement in diseaseInflammatory bowel disease 10
Sequence similaritiesBelongs to the WD repeat ATG16 family.
Contains 7 WD repeats.
modificationsProteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Cellular localizationCytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
- Information by UniProt
FormThere are 4 isoforms produced by alternative splicing.
- A16L1_HUMAN antibody
- APG16 like 1 antibody
- APG16-like 1 antibody
Lane 1 : Anti-ATG16L1 antibody (ab188642) at 0.25 µg/ml
Lane 2 : Anti-ATG16L1 antibody (ab188642) at 0.5 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 68 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
Immunocytochemical/ Immunofluorescence analysis of HeLa cells labeling ATG16L1 using ab188642 at 4 µg/ml.
Immunocytochemical analysis of HeLa cells labeling ATG16L1 using ab188642 at 2 µg/ml.
ab188642 has been referenced in 4 publications.
- Ou M et al. Emerging roles of let-7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation. Int J Mol Med 46:83-96 (2020). PubMed: 32319531
- Pellegrini D et al. Quantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease. Mol Cell Proteomics 18:1227-1241 (2019). PubMed: 30926673
- Zhang K et al. PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell Death Dis 9:312 (2018). PubMed: 29472524
- Liu H et al. Oxidative Stress Mediates Microcystin-LR-Induced Endoplasmic Reticulum Stress and Autophagy in KK-1 Cells and C57BL/6 Mice Ovaries. Front Physiol 9:1058 (2018). PubMed: 30131715