• Product name

  • Description

    Rabbit polyclonal to ATG16L1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human ATG16L1 (internal sequence). The exact sequence is proprietary.
    Database link: Q676U5

  • Positive control

    • WB: HEK-293T, A431 and HeLa whole cell lysates. IHC-P: Human ovarian carcinoma and colon carcinoma tissue; mouse liver tissue. ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab227714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.


  • Function

    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease

    Inflammatory bowel disease 10
  • Sequence similarities

    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational

    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization

    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Form

    There are 4 isoforms produced by alternative splicing.
  • Alternative names

    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all


  • All lanes : Anti-ATG16L1 antibody (ab227714) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa

    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse liver tissue stained for ATG16L1 using ab227714 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovarian carcinoma tissue stained for ATG16L1 using ab227714 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon carcinoma tissue stained for ATG16L1 using ab227714 at 1/500 dilution in immunohistochemical analysis.

  • Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 20 µM CCCP (right panel) stained for ATG16L1 (green) using ab227714 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).


ab227714 has not yet been referenced specifically in any publications.

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