Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
Involvement in disease
Inflammatory bowel disease 10
Belongs to the WD repeat ATG16 family. Contains 7 WD repeats.
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
There are 4 isoforms produced by alternative splicing.
APG16 like 1 antibody
APG16-like 1 antibody
APG16L beta antibody
ATG16 autophagy related 16 like 1 antibody
ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
Autophagy related protein 16 1 antibody
Autophagy-related protein 16-1 antibody
WD repeat domain 30 antibody
Western blot - Anti-ATG16L1 antibody (ab227714)
All lanes : Anti-ATG16L1 antibody (ab227714) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 20 µM CCCP (right panel) stained for ATG16L1 (green) using ab227714 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
has not yet been referenced specifically in any publications.
Publishing research using ab227714? Please let us know so that we can cite the reference in this datasheet.
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