Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-ATG16L1 antibody [EPR15638] - BSA and Azide free (ab232636)

Overview

  • Product name

    Anti-ATG16L1 antibody [EPR15638] - BSA and Azide free
    See all ATG16L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15638] to ATG16L1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ATG16L1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q676U5

  • Positive control

    • WB: HeLa, Jurkat and wild-type and ATG16L1 knockout HAP1 cell lysate.
  • General notes

    Ab232636 is the carrier-free version of ab187671. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232636 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

  • Function

    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease

    Inflammatory bowel disease 10
  • Sequence similarities

    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization

    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Form

    There are 4 isoforms produced by alternative splicing.
  • Alternative names

    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187671).

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ATG16L1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187671 observed at 68 and 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab187671 was shown to recognize ATG16L1 when ATG16L1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab187671 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187671).

References

ab232636 has not yet been referenced specifically in any publications.

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