Recombinant Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)
- Datasheet
- References (6)
- Protocols
Overview
-
Product name
Anti-ATG16L1 antibody [EPR15638] - N-terminal
See all ATG16L1 primary antibodies -
Description
Rabbit monoclonal [EPR15638] to ATG16L1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human ATG16L1 aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: Q676U5 -
Positive control
- HeLa, Raji, Jurkat, PC12 and NIH 3T3 cell lysates; Human prostatic hyperplasia and Mouse colon tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15638 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab187671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. -
Involvement in disease
Inflammatory bowel disease 10 -
Sequence similarities
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats. -
Post-translational
modificationsProteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. -
Cellular localization
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. - Information by UniProt
-
Database links
- Entrez Gene: 55054 Human
- Entrez Gene: 77040 Mouse
- Entrez Gene: 363278 Rat
- Omim: 610767 Human
- SwissProt: Q676U5 Human
- SwissProt: Q8C0J2 Mouse
- Unigene: 529322 Human
- Unigene: 272972 Mouse
see all -
Form
There are 4 isoforms produced by alternative splicing. -
Alternative names
- A16L1_HUMAN antibody
- APG16 like 1 antibody
- APG16-like 1 antibody
see all
Images
-
![Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)](https://www.abcam.com/ps/products/187/ab187671/Images/ab187671-260930-anti-atg16l1-antibody-epr15638-n-terminal-western-blot.jpg)
Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ATG16L1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab187671 observed at 68 and 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab187671 was shown to recognize ATG16L1 when ATG16L1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab187671 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)](https://www.abcam.com/ps/products/187/ab187671/Images/ab187671-220641-anti-atg16l1-antibody-epr15638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)](https://www.abcam.com/ps/products/187/ab187671/Images/ab187671-220640-anti-atg16l1-antibody-epr15638-n-terminal-western-blot.jpg)
Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)All lanes : Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa -
![Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)](https://www.abcam.com/ps/products/187/ab187671/Images/ab187671-220639-anti-atg16l1-antibody-epr15638-n-terminal-western-blot.jpg)
Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)All lanes : Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)](https://www.abcam.com/ps/products/187/ab187671/Images/ab187671-220642-anti-atg16l1-antibody-epr15638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). Read more (PubMed: 31309927) »
- Tian W et al. An antibody for analysis of autophagy induction. Nat Methods N/A:N/A (2019). Read more (PubMed: 31768061) »
