Overview

  • Product name

    Anti-ATG16L1 (phospho S278) antibody [EPR19016]
    See all ATG16L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19016] to ATG16L1 (phospho S278)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Mouse ATG16L1 aa 250-350 (phospho S278). The exact sequence is proprietary.
    Database link: Q8C0J2

    Synthetic peptide within Mouse ATG16L1 aa 250-350 (phospho S278). The exact sequence is proprietary.
    Database link: Q8C0J2

  • Positive control

    • WB: HEK-293 overexpressing ATG16L1 (WT) whole cell lysate.
  • General notes

    Co-immunization performed with both peptides, clone obtained by screening with peptide 1.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab195242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

Target

  • Function

    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease

    Inflammatory bowel disease 10
  • Sequence similarities

    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization

    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links

  • Form

    There are 4 isoforms produced by alternative splicing.
  • Alternative names

    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • HCT116 wild-type and ATG16L1 knockout cells were incubated with either complete media or amino acid deficient DMEM for 3 hours. 5ug of whole cell lysate were resolved by SDS-PAGE on a 6%-18% gradient gel, then transferred onto PVDF membrane. Membrane was blocked in 10X blocking buffer (Cat # ab126587) diluted in TBS solution for 30 minutes; incubated with 1:1000 primary antibody in 2.5% BSA TBST solution overnight at 4°C ; incubated with 1:15000 secondary antibody in 2% milk TBST solution for 45 minutes. Immobilon ECL was applied for 1 minute then imaged with film.

  • All lanes : Anti-ATG16L1 (phospho S278) antibody [EPR19016] (ab195242) at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with ATG16L1 (WT) expression vector containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with ATG16L1 (WT) expression vector containing a myc-His-tag®, followed by treatment with alkaline phosphatase for 1 hour, whole cell lysate
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with ATG16L1 S278A expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Dot blot analysis of ATG16L1 (phospho S278) labeled with ab195242 at 1/1,000 dilution.

    Lane 1: ATG16L1 (phospho S278) peptide;

    Lane 2: ATG16L1 non-phospho peptide;

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab195242 has not yet been referenced specifically in any publications.

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