Anti-ATG2A antibody (ab226931)
Key features and details
- Rabbit polyclonal to ATG2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG2A antibody -
Description
Rabbit polyclonal to ATG2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human ATG2A (N terminal). The exact sequence is proprietary.
Database link: Q2TAZ0 -
Positive control
- WB: U-87 MG and SK-N-SH whole cell extracts. IP: THP-1 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 212 kDa.
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IP |
1/100 - 1/571.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 212 kDa. |
IP
1/100 - 1/571. |
Target
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Sequence similarities
Belongs to the ATG2 family. - Information by UniProt
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Database links
- Entrez Gene: 107131170 Cow
- Entrez Gene: 23130 Human
- Entrez Gene: 100462717 Pig
- SwissProt: Q2TAZ0 Human
- Unigene: 370671 Human
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Alternative names
- 1810013C15Rik antibody
- A830054M12 antibody
- ATG2 autophagy related 2 homolog A (S. cerevisiae) antibody
see all
Images
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All lanes : Anti-ATG2A antibody (ab226931) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 212 kDa5% SDS-PAGE gel.
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ATG2A was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extract with 5 μg ab226931. Western blot was performed from the immunoprecipitate using ab226931.
Lane 1: Control IgG IP in THP-1 whole cell extract.
Lane 2: ab226931 IP in THP-1 whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226931 has been referenced in 1 publication.
- De Leo MG et al. WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms. Autophagy 17:3644-3670 (2021). PubMed: 33685363