Key features and details
- Rabbit polyclonal to ATG3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG3 antibody
See all ATG3 primary antibodies
DescriptionRabbit polyclonal to ATG3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ATG3 (internal sequence).
Database link: Q9NT62
- WB: HeLa whole cell lysate. ICC/IF: SK-N-BE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 35,36 kDa).|
FunctionE2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Tissue specificityWidely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Sequence similaritiesBelongs to the ATG3 family.
modificationsConjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
- Information by UniProt
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
4% formaldehyde-fixed SK-N-BE cells stained for ATG3 (green) using ab223522 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223522 (D) Merge.
Anti-ATG3 antibody (ab223522) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35,36 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
Blocking buffer: 5% skim milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.
ab223522 has not yet been referenced specifically in any publications.