Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG3 (internal sequence).
    Database link: Q9NT62

  • Positive control

    • WB: HeLa whole cell lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 35,36 kDa).
ICC/IF 1/100.

Target

  • Function

    E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
  • Tissue specificity

    Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
  • Sequence similarities

    Belongs to the ATG3 family.
  • Post-translational
    modifications

    Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610016C12Rik antibody
    • Apg 3 antibody
    • APG3 antibody
    • APG3 autophagy 3 like antibody
    • APG3 like antibody
    • APG3, S. cerevisiae, homolog of antibody
    • APG3-like antibody
    • APG3L antibody
    • Apg3p antibody
    • ATG 3 antibody
    • ATG3 antibody
    • ATG3 autophagy related 3 homolog antibody
    • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
    • ATG3_HUMAN antibody
    • Autophagy 3, S. cerevisiae, homolog of antibody
    • Autophagy Apg3p/Aut1p like antibody
    • autophagy related 3 antibody
    • Autophagy related protein 3 antibody
    • Autophagy-related protein 3 antibody
    • DKFZp564M1178 antibody
    • FLJ22125 antibody
    • hApg3 antibody
    • MGC15201 antibody
    • OTTHUMP00000214547 antibody
    • OTTHUMP00000214548 antibody
    • PC3 96 antibody
    • Protein PC3-96 antibody
    • Ubiquitin-like-conjugating enzyme ATG3 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for ATG3 (green) using ab223522 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT. 

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223522 (D) Merge.

  • Anti-ATG3 antibody (ab223522) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 35,36 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 5% skim milk in TBST.

    Primary and secondary antibodies were incubated for 1 hour at RT.

References

ab223522 has not yet been referenced specifically in any publications.

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