Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ATG3 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NT62

  • Positive control

    • WB: HeLa whole cell extract; Jurkat, Raji, PC-12, Rat2, C8D30, NIH/3T3, BCL-1, RAW 264.7, K 562, THP-1 and HL-60 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab228749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.

Target

  • Function

    E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
  • Tissue specificity

    Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
  • Sequence similarities

    Belongs to the ATG3 family.
  • Post-translational
    modifications

    Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610016C12Rik antibody
    • Apg 3 antibody
    • APG3 antibody
    • APG3 autophagy 3 like antibody
    • APG3 like antibody
    • APG3, S. cerevisiae, homolog of antibody
    • APG3-like antibody
    • APG3L antibody
    • Apg3p antibody
    • ATG 3 antibody
    • ATG3 antibody
    • ATG3 autophagy related 3 homolog antibody
    • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
    • ATG3_HUMAN antibody
    • Autophagy 3, S. cerevisiae, homolog of antibody
    • Autophagy Apg3p/Aut1p like antibody
    • autophagy related 3 antibody
    • Autophagy related protein 3 antibody
    • Autophagy-related protein 3 antibody
    • DKFZp564M1178 antibody
    • FLJ22125 antibody
    • hApg3 antibody
    • MGC15201 antibody
    • OTTHUMP00000214547 antibody
    • OTTHUMP00000214548 antibody
    • PC3 96 antibody
    • Protein PC3-96 antibody
    • Ubiquitin-like-conjugating enzyme ATG3 antibody
    see all

Images

  • All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Apg3 shRNA-transfected HeLa whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-ATG3 antibody (ab228749) at 1/2000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution

    Lane 1 : C8D30 whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : BCL-1 whole cell lysate
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-ATG3 antibody (ab228749) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

References

ab228749 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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