Recombinant Anti-ATG3 antibody [EPR4801] (ab108251)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-ATG3 antibody [EPR4801]
See all ATG3 primary antibodies -
DescriptionRabbit monoclonal [EPR4801] to ATG3
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human ATG3 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: K562, HL-60, HeLa or Jurkat whole cell lysate (ab7899). IHC-P: Human muscle tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4801
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). | |
| IHC-P | 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/2000. | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionE2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
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Tissue specificityWidely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
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Sequence similaritiesBelongs to the ATG3 family.
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Post-translational
modificationsConjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 64422 Human
- Entrez Gene: 67841 Mouse
- Entrez Gene: 171415 Rat
- Omim: 609606 Human
- SwissProt: Q9NT62 Human
- SwissProt: Q9CPX6 Mouse
- SwissProt: Q6AZ50 Rat
- Unigene: 477126 Human
see all -
Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
see all
Images
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![Western blot - Anti-ATG3 antibody [EPR4801] (ab108251)](https://www.abcam.com/ps/products/108/ab108251/Images/ab108251-120762-anti-atg3-antibody-epr4801-western-blot.jpg)
Western blot - Anti-ATG3 antibody [EPR4801] (ab108251)All lanes : Anti-ATG3 antibody [EPR4801] (ab108251) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Frozen sections) - Anti-ATG3 antibody [EPR4801] (ab108251)](https://www.abcam.com/ps/products/108/ab108251/Images/ab108251-211212-anti-atg3-antibody-epr4801-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-ATG3 antibody [EPR4801] (ab108251)This image is courtesy of an anonymous Abreviewab108251 staining ATG3 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [EPR4801] (ab108251)](https://www.abcam.com/ps/products/108/ab108251/Images/ab108251-120760-anti-atg3-antibody-epr4801-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATG3 antibody [EPR4801] (ab108251)Immunofluorescent staining of HeLa cells using ab108251 at 1/100. -
![Flow Cytometry - Anti-ATG3 antibody [EPR4801] (ab108251)](https://www.abcam.com/ps/products/108/ab108251/Images/ab108251-280406-anti-atg3-antibody-epr4801-flow-cytometry.jpg)
Flow Cytometry - Anti-ATG3 antibody [EPR4801] (ab108251)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATG3 (red) with ab108251 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [EPR4801] (ab108251)](https://www.abcam.com/ps/products/108/ab108251/Images/ab108251-120761-anti-atg3-antibody-epr4801-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG3 antibody [EPR4801] (ab108251)ab108251, at a 1/100 dilution, staining Human ATG3 in muscle, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Qiu S et al. The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells. Biomed Pharmacother 109:1841-1850 (2019). Read more (PubMed: 30551439) »
- Agrotis A et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). Read more (PubMed: 30661429) »
