Overview

  • Product name

    Anti-ATG3 antibody [EPR4802]
    See all ATG3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4802] to ATG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATG3 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • K562, HeLa or Jurkat cell lysates (WB) Permeabilized Jurkat cells (FC)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
    • Tissue specificity

      Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
    • Sequence similarities

      Belongs to the ATG3 family.
    • Post-translational
      modifications

      Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2610016C12Rik antibody
      • Apg 3 antibody
      • APG3 antibody
      • APG3 autophagy 3 like antibody
      • APG3 like antibody
      • APG3, S. cerevisiae, homolog of antibody
      • APG3-like antibody
      • APG3L antibody
      • Apg3p antibody
      • ATG 3 antibody
      • ATG3 antibody
      • ATG3 autophagy related 3 homolog antibody
      • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
      • ATG3_HUMAN antibody
      • Autophagy 3, S. cerevisiae, homolog of antibody
      • Autophagy Apg3p/Aut1p like antibody
      • autophagy related 3 antibody
      • Autophagy related protein 3 antibody
      • Autophagy-related protein 3 antibody
      • DKFZp564M1178 antibody
      • FLJ22125 antibody
      • hApg3 antibody
      • MGC15201 antibody
      • OTTHUMP00000214547 antibody
      • OTTHUMP00000214548 antibody
      • PC3 96 antibody
      • Protein PC3-96 antibody
      • Ubiquitin-like-conjugating enzyme ATG3 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).
    • All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Agrotis A  et al. Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. J Biol Chem 294:12610-12621 (2019). Read more (PubMed: 31315929) »
    • Chen L  et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). Read more (PubMed: 29212196) »
    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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