Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG4A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ATG4A aa 102-113. From the mid-protein.
    Sequence:

    EKQKEQPKEYQR


    Database link: Q8WYN0

  • Positive control

    • WB: Rat pancreas cell lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 37,39, 42, 45 kDa).

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
  • Tissue specificity

    Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI627006 antibody
    • APG4 autophagy 4 homolog A antibody
    • Apg4a antibody
    • ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody
    • ATG4 autophagy related 4 homolog A antibody
    • ATG4A antibody
    • ATG4A_HUMAN antibody
    • Atg4al antibody
    • AUT like 2 cysteine endopeptidase antibody
    • AUT-like 2 cysteine endopeptidase antibody
    • Autl2 antibody
    • Autophagin 2 antibody
    • Autophagin-2 antibody
    • Autophagy related 4A cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 2 antibody
    • Autophagy related protein 4 homolog A antibody
    • Autophagy-related cysteine endopeptidase 2 antibody
    • Autophagy-related protein 4 homolog A antibody
    • AV169859 antibody
    • Cysteine protease ATG4A antibody
    • hAPG4A antibody
    • MGC107179 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for ATG4A (green) using ab223374 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223374 (D) Merge.

  • Anti-ATG4A antibody (ab223374) at 1/1000 dilution + rat pancreas cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 37,39, 42, 45 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 5% skimmed milk in TBST.

    Primary and secondary antibodies were incubated for 1 hour at RT.

References

ab223374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223374.
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