Key features and details
- Rabbit polyclonal to ATG4A
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ATG4A antibody
See all ATG4A primary antibodies
DescriptionRabbit polyclonal to ATG4A
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan
- WB: Rat pancreas cell lysate. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 37,39, 42, 45 kDa).|
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Tissue specificityWidely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Sequence similaritiesBelongs to the peptidase C54 family.
- Information by UniProt
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
4% formaldehyde-fixed SK-N-BE cells stained for ATG4A (green) using ab223374 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223374 (D) Merge.
Anti-ATG4A antibody (ab223374) at 1/1000 dilution + rat pancreas cell lysate at 15 µg
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37,39, 42, 45 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
Blocking buffer: 5% skimmed milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.
ab223374 has not yet been referenced specifically in any publications.