Recombinant Anti-ATG4A antibody [EPR4122] (ab108322)
![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-453261-anti-atg4a-antibody-epr4122-western-blot-hela-wt-atg4a-knockout-hela-hepg2-daudi-human.jpg "Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4122] to ATG4A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATG4A antibody [EPR4122]
See all ATG4A primary antibodies -
Description
Rabbit monoclonal [EPR4122] to ATG4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal liver, HeLa, Daudi, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval. |
Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. -
Tissue specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 115201 Human
- Entrez Gene: 666468 Mouse
- Omim: 300663 Human
- SwissProt: Q8WYN0 Human
- SwissProt: Q8C9S8 Mouse
- Unigene: 8763 Human
- Unigene: 102230 Mouse
- Unigene: 393320 Mouse
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Alternative names
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
see all
Images
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Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG4A knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108322 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab108322 was shown to react with ATG4A in wild-type HeLa cells in Western blot with loss of signal observed in ATG4A knockout cell line ab265738 (ATG4A knockout cell lysate ab257846). Wild-type HeLa and ATG4A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108322 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120888-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120887-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120889-anti-atg4a-antibody-epr4122-western-blot.jpg)
Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Jurkat lysate
Lane 4 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-4-benefits-of-recombinant-antibodies.png)
Anti-ATG4A antibody [EPR4122] (ab108322)
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab108322 has been referenced in 8 publications.
- Zhu K et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291
- Zhang Z et al. RNA-binding protein ZFP36/TTP protects against ferroptosis by regulating autophagy signaling pathway in hepatic stellate cells. Autophagy 16:1482-1505 (2020). PubMed: 31679460
- Xu C et al. miR-433 Inhibits Neuronal Growth and Promotes Autophagy in Mouse Hippocampal HT-22 Cell Line. Front Pharmacol 11:536913 (2020). PubMed: 33381022
- Xie X et al. Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells. Cell Death Dis 10:94 (2019). PubMed: 30700698
- Lian WS et al. Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR. Cell Death Dis 9:938 (2018). PubMed: 30224697
- Ni Z et al. AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Autophagy 14:685-701 (2018). PubMed: 29165041
- Wang D et al. Diabetes Exacerbates Myocardial Ischemia/Reperfusion Injury by Down-Regulation of MicroRNA and Up-Regulation of O-GlcNAcylation. JACC Basic Transl Sci 3:350-362 (2018). PubMed: 30062222
- Su H et al. Upregulation of ATG4A promotes osteosarcoma cell epithelial-to-mesenchymal transition through the Notch signaling pathway. Int J Clin Exp Pathol 10:7975-7982 (2017). PubMed: 31966649
