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  1. Link

    atg4a-antibody-epr4122-ab108322.pdf

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Cancer Invasion/microenvironment ECM Extracellular matrix Other
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ATG4A antibody [EPR4122] (ab108322)

  • Datasheet
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Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
  • Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
  • Anti-ATG4A antibody [EPR4122] (ab108322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4122] to ATG4A
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
Recombinant Human ATG4A protein (ab172826)

View more associated products

Overview

  • Product name

    Anti-ATG4A antibody [EPR4122]
    See all ATG4A primary antibodies
  • Description

    Rabbit monoclonal [EPR4122] to ATG4A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal liver, HeLa, Daudi, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4122
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products

Associated products

  • Alternative Versions

    • Anti-ATG4A antibody [EPR4122] - BSA and Azide free (ab247630)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ATG4A knockout HeLa cell line (ab265738)
  • KO cell lysates

    • Human ATG4A knockout HeLa cell lysate (ab257846)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ATG4A protein (ab172826)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval.

Application notes
Is unsuitable for Flow Cyt or ICC.

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
  • Tissue specificity

    Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q8WYN0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 115201 Human
    • Entrez Gene: 666468 Mouse
    • Omim: 300663 Human
    • SwissProt: Q8WYN0 Human
    • SwissProt: Q8C9S8 Mouse
    • Unigene: 8763 Human
    • Unigene: 102230 Mouse
    • Unigene: 393320 Mouse
    • Alternative names

      • AI627006 antibody
      • APG4 autophagy 4 homolog A antibody
      • Apg4a antibody
      • ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody
      • ATG4 autophagy related 4 homolog A antibody
      • ATG4A antibody
      • ATG4A_HUMAN antibody
      • Atg4al antibody
      • AUT like 2 cysteine endopeptidase antibody
      • AUT-like 2 cysteine endopeptidase antibody
      • Autl2 antibody
      • Autophagin 2 antibody
      • Autophagin-2 antibody
      • Autophagy related 4A cysteine peptidase antibody
      • Autophagy related cysteine endopeptidase 2 antibody
      • Autophagy related protein 4 homolog A antibody
      • Autophagy-related cysteine endopeptidase 2 antibody
      • Autophagy-related protein 4 homolog A antibody
      • AV169859 antibody
      • Cysteine protease ATG4A antibody
      • hAPG4A antibody
      • MGC107179 antibody
      see all

    Images

    • Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
      Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
      All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ATG4A knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 45 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab108322 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab108322 was shown to react with ATG4A in wild-type HeLa cells in Western blot with loss of signal observed in ATG4A knockout cell line ab265738 (ATG4A knockout cell lysate ab257846). Wild-type HeLa and ATG4A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108322 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
      Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
      All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Anti-ATG4A antibody [EPR4122] (ab108322)
      Anti-ATG4A antibody [EPR4122] (ab108322)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (8)

    Publishing research using ab108322? Please let us know so that we can cite the reference in this datasheet.

    ab108322 has been referenced in 8 publications.

    • Zhu K  et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291
    • Zhang Z  et al. RNA-binding protein ZFP36/TTP protects against ferroptosis by regulating autophagy signaling pathway in hepatic stellate cells. Autophagy 16:1482-1505 (2020). PubMed: 31679460
    • Xu C  et al. miR-433 Inhibits Neuronal Growth and Promotes Autophagy in Mouse Hippocampal HT-22 Cell Line. Front Pharmacol 11:536913 (2020). PubMed: 33381022
    • Xie X  et al. Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells. Cell Death Dis 10:94 (2019). PubMed: 30700698
    • Lian WS  et al. Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR. Cell Death Dis 9:938 (2018). PubMed: 30224697
    • Ni Z  et al. AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Autophagy 14:685-701 (2018). PubMed: 29165041
    • Wang D  et al. Diabetes Exacerbates Myocardial Ischemia/Reperfusion Injury by Down-Regulation of MicroRNA and Up-Regulation of O-GlcNAcylation. JACC Basic Transl Sci 3:350-362 (2018). PubMed: 30062222
    • Su H  et al. Upregulation of ATG4A promotes osteosarcoma cell epithelial-to-mesenchymal transition through the Notch signaling pathway. Int J Clin Exp Pathol 10:7975-7982 (2017). PubMed: 31966649

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