Recombinant
RabMAb

Anti-ATG4A antibody [EPR4122] (ab108322)

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Overview

  • Product name
    Anti-ATG4A antibody [EPR4122]
    See all ATG4A primary antibodies
  • Description
    Rabbit monoclonal [EPR4122] to ATG4A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATG4A. The exact sequence is proprietary.

  • Positive control
    • Fetal liver, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform antigen retrieval.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function
      Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
    • Tissue specificity
      Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
    • Sequence similarities
      Belongs to the peptidase C54 family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
      • Alternative names
        • AI627006 antibody
        • APG4 autophagy 4 homolog A antibody
        • Apg4a antibody
        • ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody
        • ATG4 autophagy related 4 homolog A antibody
        • ATG4A antibody
        • ATG4A_HUMAN antibody
        • Atg4al antibody
        • AUT like 2 cysteine endopeptidase antibody
        • AUT-like 2 cysteine endopeptidase antibody
        • Autl2 antibody
        • Autophagin 2 antibody
        • Autophagin-2 antibody
        • Autophagy related 4A cysteine peptidase antibody
        • Autophagy related cysteine endopeptidase 2 antibody
        • Autophagy related protein 4 homolog A antibody
        • Autophagy-related cysteine endopeptidase 2 antibody
        • Autophagy-related protein 4 homolog A antibody
        • AV169859 antibody
        • Cysteine protease ATG4A antibody
        • hAPG4A antibody
        • MGC107179 antibody
        see all

      Images

      • Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
        Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)
        All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution

        Lane 1 : Fetal liver lysate
        Lane 2 : HepG2 lysate
        Lane 3 : Jurkat lysate
        Lane 4 : Fetal brain lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
        Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)
        Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.

      References

      ab108322 has not yet been referenced specifically in any publications.

      Publishing research using ab108322? Please let us know so that we can cite the reference in this datasheet.

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