Recombinant Anti-ATG4A antibody [EPR4122] (ab108322)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-ATG4A antibody [EPR4122]
See all ATG4A primary antibodies -
Description
Rabbit monoclonal [EPR4122] to ATG4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ATG4A. The exact sequence is proprietary.
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Positive control
- Fetal liver, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 45 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval. |
Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. -
Tissue specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 115201 Human
- Entrez Gene: 666468 Mouse
- Omim: 300663 Human
- SwissProt: Q8WYN0 Human
- SwissProt: Q8C9S8 Mouse
- Unigene: 8763 Human
- Unigene: 102230 Mouse
- Unigene: 393320 Mouse
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Alternative names
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
see all
Images
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![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120889-anti-atg4a-antibody-epr4122-western-blot.jpg)
Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Jurkat lysate
Lane 4 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120888-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120887-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Xie X et al. Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells. Cell Death Dis 10:94 (2019). Read more (PubMed: 30700698) »
- Lian WS et al. Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR. Cell Death Dis 9:938 (2018). Read more (PubMed: 30224697) »
