Recombinant Anti-ATG4A antibody [EPR4122] (ab108322)
![Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120889-anti-atg4a-antibody-epr4122-western-blot.jpg "Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4122] to ATG4A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATG4A antibody [EPR4122]
See all ATG4A primary antibodies -
Description
Rabbit monoclonal [EPR4122] to ATG4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ATG4A. The exact sequence is proprietary.
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Positive control
- Fetal liver, HepG2, Jurkat and fetal brain lysates. Human brain tissue and Human skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 45 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval. |
Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. -
Tissue specificity
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 115201 Human
- Entrez Gene: 666468 Mouse
- Omim: 300663 Human
- SwissProt: Q8WYN0 Human
- SwissProt: Q8C9S8 Mouse
- Unigene: 8763 Human
- Unigene: 102230 Mouse
- Unigene: 393320 Mouse
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Alternative names
- AI627006 antibody
- APG4 autophagy 4 homolog A antibody
- Apg4a antibody
see all
Images
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Western blot - Anti-ATG4A antibody [EPR4122] (ab108322)All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Jurkat lysate
Lane 4 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120888-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-120887-anti-atg4a-antibody-epr4122-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4A antibody [EPR4122] (ab108322)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-ATG4A antibody [EPR4122] (ab108322)](https://www.abcam.com/ps/products/108/ab108322/Images/ab108322-4-benefits-of-recombinant-antibodies.png)
Anti-ATG4A antibody [EPR4122] (ab108322)
Protocols
Datasheets and documents
References (6)
ab108322 has been referenced in 6 publications.
- Zhu K et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291
- Xie X et al. Downregulation of G2/mitotic-specific cyclinB1 triggers autophagy via AMPK-ULK1-dependent signal pathway in nasopharyngeal carcinoma cells. Cell Death Dis 10:94 (2019). PubMed: 30700698
- Lian WS et al. Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR. Cell Death Dis 9:938 (2018). PubMed: 30224697
- Ni Z et al. AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Autophagy 14:685-701 (2018). PubMed: 29165041
- Wang D et al. Diabetes Exacerbates Myocardial Ischemia/Reperfusion Injury by Down-Regulation of MicroRNA and Up-Regulation of O-GlcNAcylation. JACC Basic Transl Sci 3:350-362 (2018). PubMed: 30062222
- Su H et al. Upregulation of ATG4A promotes osteosarcoma cell epithelial-to-mesenchymal transition through the Notch signaling pathway. Int J Clin Exp Pathol 10:7975-7982 (2017). PubMed: 31966649
