Anti-ATG4B antibody (ab223364)
Key features and details
- Rabbit polyclonal to ATG4B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ATG4B antibody
See all ATG4B primary antibodies -
Description
Rabbit polyclonal to ATG4B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Human ATG4B aa 50-150.
Database link: Q9Y4P1 -
Positive control
- WB: Mouse brain cell lysate. ICC/IF: SK-N-BE cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
|
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WB |
1/1000.
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Notes |
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ICC/IF
1/100. |
WB
1/1000. |
Target
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Function
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. -
Tissue specificity
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. -
Sequence similarities
Belongs to the peptidase C54 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23192 Human
- Entrez Gene: 66615 Mouse
- Omim: 611338 Human
- SwissProt: Q9Y4P1 Human
- SwissProt: Q8BGE6 Mouse
- Unigene: 283610 Human
- Unigene: 29087 Mouse
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Alternative names
- APG4 autophagy 4 homolog B antibody
- APG4B antibody
- ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
see all
Images
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4% formaldehyde-fixed SK-N-BE (human) cells stained for ATG4B (green) using ab223364 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223364 (D) Merge.
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Anti-ATG4B antibody (ab223364) at 1/1000 dilution + Mouse brain cell lysate at 15 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking buffer: 5% skimmed milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223364 has not yet been referenced specifically in any publications.