Key features and details
- Rabbit polyclonal to ATG4B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATG4B antibody
See all ATG4B primary antibodies
DescriptionRabbit polyclonal to ATG4B
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- WB: Mouse brain cell lysate. ICC/IF: SK-N-BE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Sequence similaritiesBelongs to the peptidase C54 family.
- Information by UniProt
- APG4 autophagy 4 homolog B antibody
- APG4B antibody
- ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
4% formaldehyde-fixed SK-N-BE (human) cells stained for ATG4B (green) using ab223364 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223364 (D) Merge.
Anti-ATG4B antibody (ab223364) at 1/1000 dilution + Mouse brain cell lysate at 15 µg
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
Blocking buffer: 5% skimmed milk in TBST.
Primary and secondary antibodies were incubated for 1 hour at RT.
ab223364 has not yet been referenced specifically in any publications.