Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human ATG4B aa 94-106.
    Sequence:

    RDWRWTQRKRQPD


    Database link: Q9Y4P1

  • Positive control

    • WB: Mouse brain cell lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000.

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity

    Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related 4B cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for ATG4B (green) using ab223364 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223364 (D) Merge.

     

  • Anti-ATG4B antibody (ab223364) at 1/1000 dilution + Mouse brain cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 5% skimmed milk in TBST.

    Primary and secondary antibodies were incubated for 1 hour at RT.

References

ab223364 has not yet been referenced specifically in any publications.

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